The quarterfinal field for the 2016 US Open Cup is set after the final set of Round of 16 matches were played on Wednesday night.
The elite eight pairings feature two sets of MLS rivals going head-to-head, an MLS Eastern Conference grudge match and the Chicago Fire hosting the lone non-MLS team left standing:
| 2016 US Open Cup Quarterfinals – Wednesday, July 20 | |||
|---|---|---|---|
| 7:30 ET | New England Revolution vs. Philadelphia Union | Harvard University | Boston, Mass. |
| 8:30 ET | Chicago Fire vs. Fort Lauderdale Strikers | Toyota Park | Bridgeview, Ill. |
| 8:30 ET | Houston Dynamo vs. FC Dallas | BBVA Compass Stadium | Houston, Tex. |
| 10:30 ET | LA Galaxy vs. Seattle Sounders | StubHub Center | Carson, Calif. |
